9H0N

Fucosylated Lacto-N-biose binding protein from Bifidobacterium longum subsp. infantis in complex with Galacto-N-biose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	2.075
Spacegroup name	P 21 21 21
Unit cell lengths	52.100, 53.250, 149.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.410 - 1.400
R-factor	0.1689
Rwork	0.168
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.410	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	79467	1956
<I/σ(I)>	34.34
Completeness [%]	95.7	67.31
Redundancy	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M NaCl, 0.1 M Tris pH 7.0, 30% PEG3000 + TCEP