9H0C
Crystal structure of BiP ATPase domain in complex with CDNF C-terminal domain at 1.65 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999870 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.522, 80.612, 102.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.310 - 1.650 |
| R-factor | 0.18161 |
| Rwork | 0.181 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.310 | 1.678 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.108 | 1.129 |
| Rmeas | 0.123 | 1.320 |
| Rpim | 0.058 | 0.671 |
| Number of reflections | 50649 | 2315 |
| <I/σ(I)> | 8.1 | 1.3 |
| Completeness [%] | 99.2 | 92.4 |
| Redundancy | 4.3 | 3.6 |
| CC(1/2) | 0.995 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES pH 7.5, 200 mM NaCl, 25% (w/v) PEG3350 |
