9GZQ
Structure of ForCE lacking the Helical Membrane Plug-in (HMP; DUF1641)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.73404 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 215.909, 215.909, 215.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.060 - 3.626 |
| R-factor | 0.2821 |
| Rwork | 0.280 |
| R-free | 0.31950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 152.700 | 3.720 |
| High resolution limit [Å] | 3.626 | 3.630 |
| Rmerge | 0.159 | |
| Number of reflections | 54862 | 4099 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18M tri-Ammonium citrate, 2.2M Ammonium sulfate |
