9GZK
Crystal structure of the L. monocytogenes RmlT in complex with 4-RboP-(CH2)6NH2
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.318, 290.759, 91.031 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 42.890 - 2.520 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.424 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.071 | 1.053 |
| Rmeas | 0.084 | 1.242 |
| Rpim | 0.045 | 0.655 |
| Number of reflections | 147299 | 7280 |
| <I/σ(I)> | 13.2 | 1.4 |
| Completeness [%] | 99.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris propane pH 7.0 0.2 M K/Na tartrate tetrahydrate 17.5% (w/v) PEG 3,350 |
