9GZ5
X-ray structure of Leptospira interrogans Histone deacetylase 11 (HDAC11) in complex with cis-5-dodecenoic acid.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.265, 61.744, 49.481 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 48.750 - 1.550 |
| R-factor | 0.17438 |
| Rwork | 0.173 |
| R-free | 0.20071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.116 | 1.681 |
| Rpim | 0.048 | 0.621 |
| Number of reflections | 40698 | 1844 |
| <I/σ(I)> | 13 | 1.1 |
| Completeness [%] | 98.7 | 91.2 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.997 | 0.451 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 1 protein:2 precipitant 100 mM HEPES pH 7.4, 200 mM MgCl2, 23% PEG3350 |
