9GYS
X-ray structure of the adduct formed upon reaction of RNase A with [Ru2(D-p-FPhF)(O2CCH3)2(O2CO)] complex
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.540, 32.600, 72.350 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 50.270 - 1.740 |
| Rwork | 0.192 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.581 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.270 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.052 | 0.466 |
| Number of reflections | 23214 | 1137 |
| <I/σ(I)> | 11.6 | 2.1 |
| Completeness [%] | 94.4 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.997 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 22% PEG4K, 10 mM sodium citrate buffer at pH 5.1 |
