9GYR
Ferredoxin CNF labelled, oxidised state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-08 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.77 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.834, 61.532, 58.843 |
| Unit cell angles | 90.00, 119.24, 90.00 |
Refinement procedure
| Resolution | 39.420 - 1.100 |
| R-factor | 0.1439 |
| Rwork | 0.131 |
| R-free | 0.14420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.748 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.064 | 0.804 |
| Rpim | 0.029 | 0.376 |
| Number of reflections | 148067 | 7311 |
| <I/σ(I)> | 11 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.997 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Na K phosphate pH 7-8 2.6-3.6 M Ammonium sulfate |
