9GYQ
Crystal structure of human Histidine Triad Nucleotide-Binding Protein 1 in complex with KV30
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-18 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.011, 46.467, 63.839 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 18.030 - 2.001 |
| Rwork | 0.141 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.631 |
| Data reduction software | CrysalisPro (43.100a) |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.030 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.206 |
| Rmeas | 0.080 | 0.251 |
| Rpim | 0.045 | 0.141 |
| Number of reflections | 15270 | 1147 |
| <I/σ(I)> | 12.3 | 4.8 |
| Completeness [%] | 98.2 | 99.5 |
| Redundancy | 2.7 | 2.8 |
| CC(1/2) | 0.996 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 10% w/v PEG4000, 0.1 M sodium cacodylate pH 6.0 |
