Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-07 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.62 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.050, 62.050, 85.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.232 - 1.190 |
| Rwork | 0.156 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.358 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.232 | 1.220 |
| High resolution limit [Å] | 1.190 | 1.200 |
| Rmerge | 0.100 | 3.200 |
| Rpim | 0.020 | 0.930 |
| Number of reflections | 54217 | 2631 |
| <I/σ(I)> | 14.8 | 0.5 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 25.3 | 24.3 |
| CC(1/2) | 1.000 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Na K Phosphate pH 7-8, 26-36 M Ammonium Sulphate |
