9GYD
Ferredoxin Wild-type -As-isolated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.301, 51.410, 61.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.400 - 0.920 |
| R-factor | 0.1272 |
| Rwork | 0.127 |
| R-free | 0.13840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.291 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 0.940 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.049 | 1.190 |
| Rpim | 0.014 | 0.370 |
| Number of reflections | 128152 | 3289 |
| <I/σ(I)> | 18.2 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 10.1 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100 mM Na, K phosphate, 2.6-3.6 M Ammonium phosphate |
