9GXF

Crystal structure of Lotus japonicus CHIP13 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	58.739, 91.902, 92.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.490 - 1.640
R-factor	0.177
Rwork	0.176
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	1.680
High resolution limit [Å]	1.640	1.640
Rmeas	0.092	2.612
Rpim	0.025	0.701
Number of reflections	61671	4077
<I/σ(I)>	14.61	0.89
Completeness [%]	99.8
Redundancy	13.5
CC(1/2)	0.999	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	0.1 M NaAc . 3H2O pH 4.5 23% PEG-3350