9GVR

mEos4b-L93M photoconvertible fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	39.278, 57.300, 102.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.250 - 1.860
R-factor	0.1718
Rwork	0.168
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.774
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.040	1.930
High resolution limit [Å]	1.860	1.860
Rmeas	0.237
Rpim	0.081	0.442
Number of reflections	20037	1913
<I/σ(I)>	8.92	2.46
Completeness [%]	99.7	97.8
Redundancy	8.4	8.8
CC(1/2)	0.993	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M HEPES pH 8.5, 32 % PEG 1000