9GTZ

Xenopus tropicalis Interleukin Enhancer-Binding Factor 3 (ILF3) and Interleukin Enhancer-Binding Factor 2 (ILF2) heterodimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-09-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	80.971, 80.971, 230.358
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.820 - 2.531
Rwork	0.220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419:)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.820	2.560
High resolution limit [Å]	2.531	2.531
Rmerge	0.144
Number of reflections	30494	30494
<I/σ(I)>	10.2
Completeness [%]	100.0
Redundancy	1
CC(1/2)		0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES pH 7.5, 25% PEG 3,350