Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.698, 93.482, 125.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.770 - 2.145 |
| R-factor | 0.2513 |
| Rwork | 0.249 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.8 (8-JUN-2022) PACIOREK) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.770 | 2.327 |
| High resolution limit [Å] | 2.145 | 2.145 |
| Rmerge | 0.072 | 1.303 |
| Rmeas | 0.078 | 1.409 |
| Rpim | 0.030 | 0.531 |
| Total number of observations | 8225 | |
| Number of reflections | 23604 | 1180 |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 74.8 | |
| Redundancy | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4K 21%w/v pH 0, MES 0.1M pH 6.5, NaCl 0.3M pH 0 |
