9GTX
Structural and functional analysis of the Helicobacter pylori lipoprotein chaperone LolA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.749, 65.461, 60.807 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 44.240 - 2.040 |
| R-factor | 0.2029 |
| Rwork | 0.200 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.869 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1.5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.240 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.142 | 1.113 |
| Rmeas | 0.154 | 1.200 |
| Rpim | 0.059 | 0.447 |
| Total number of observations | 142035 | 14420 |
| Number of reflections | 20710 | 2017 |
| <I/σ(I)> | 9.2 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 0.996 | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 50% pentaerythriol propoxylate 0.1 M Tris pH 8 |
