9GR8
Crystal structure of the N-terminal kinase domain from Saccharomyces cerevisiae Vip1 in complex with ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000036 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.675, 83.172, 51.768 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 43.860 - 1.180 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.860 | 1.250 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmeas | 0.104 | 2.020 |
| Number of reflections | 220120 | 36388 |
| <I/σ(I)> | 13.3 | 1.2 |
| Completeness [%] | 97.5 | 93.4 |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 1.000 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 23 % [v/v] PEG 3,350, 0.1 M citric acid / BIS-Tris propane pH 6.4 |
