9GQT

influenza neuraminidase hybrid N1/09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-07
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	0.6199
Spacegroup name	P 4
Unit cell lengths	92.089, 92.089, 146.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.120 - 1.940
R-factor	0.1832
Rwork	0.183
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.154
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	1.970
High resolution limit [Å]	1.940	1.940
Number of reflections	90312	4440
<I/σ(I)>	4.1
Completeness [%]	99.9
Redundancy	13.9
CC(1/2)	0.983	0.282

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	25% w/v pentaerythritol ethoxylate 797, 100 mM MES pH 6.5, 50 mM magnesium chloride