9GQS

Teth514_1788 1,2-beta-oligomannan phosphorylase in complex with mannose (+1) and phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9677
Spacegroup name	P 32 2 1
Unit cell lengths	137.269, 137.269, 169.384
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.972 - 2.500
Rwork	0.150
R-free	0.17210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.112
Number of reflections	64153	9258
<I/σ(I)>	6.4
Completeness [%]	99.8
Redundancy	5.7
CC(1/2)	0.996	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.1 M Sodium cacodylate trihydrate pH 6.5, 1.0 M Sodium citrate tribasic dihydrate