9GQQ

influenza neuraminidase N1/19 hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-18
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	6199
Spacegroup name	C 2 2 21
Unit cell lengths	115.412, 234.367, 115.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.690 - 1.780
R-factor	0.1897
Rwork	0.189
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.705
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.314	1.981
High resolution limit [Å]	1.775	1.775
Number of reflections	58546	2927
<I/σ(I)>	5.4
Completeness [%]	94.2
Redundancy	13.4
CC(1/2)	0.989	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	5% PGA_LM, 30% PEG550 MME, 0.1M Tris pH 7.8