9GQM
Structural characterization of glucose- and fucose-binding sites in human RNase2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.885, 52.829, 56.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.600 - 1.010 |
| R-factor | 0.1359 |
| Rwork | 0.135 |
| R-free | 0.15240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.939 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 1.071 |
| High resolution limit [Å] | 1.010 | 1.036 |
| Number of reflections | 56650 | 2832 |
| <I/σ(I)> | 1.45 | |
| Completeness [%] | 86.9 | 30.4 |
| Redundancy | 5.4 | 1.7 |
| CC(1/2) | 0.998 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M Sodium acetate trihydrate, pH 7.0, 12% w/v Polyethylene glycol 3,350 |
