9GQ0
Structural characterization of fucose-binding site in human RNase2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.704, 52.553, 56.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.480 - 1.040 |
| R-factor | 0.1308 |
| Rwork | 0.131 |
| R-free | 0.14150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.480 | 1.071 |
| High resolution limit [Å] | 1.036 | 1.036 |
| Number of reflections | 55078 | 2832 |
| <I/σ(I)> | 1.45 | |
| Completeness [%] | 91.8 | 47.2 |
| Redundancy | 6.8 | 2.3 |
| CC(1/2) | 0.999 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.18M Formate, PH8.2, 0.1M Bis-Tris propane, 15% w/v Polyethylene glycol 3,350 |
