9GPJ

Structure of UP1 S4DS6D phosphomimetic mutant in complex with human telomeric repeat DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9999
Spacegroup name	P 43 21 2
Unit cell lengths	51.425, 51.425, 172.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.140 - 1.530
R-factor	0.1855
Rwork	0.184
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.938
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.360	1.560
High resolution limit [Å]	1.530	1.530
Number of reflections	36066	1755
<I/σ(I)>	27.4	3.2
Completeness [%]	100.0	99.9
Redundancy	14.07
CC(1/2)	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1 M Tris, pH 8.50, 1.7 M Ammonium Phosphate Dibasic, 15 % (v/v) glycerol