9GP5
Homodimer of BACH1 F9A mutant BTB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.084, 70.334, 65.246 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.900 |
| R-factor | 0.19624 |
| Rwork | 0.195 |
| R-free | 0.22555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.695 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.330 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.630 |
| Rmeas | 0.092 | 0.697 |
| Rpim | 0.039 | 0.292 |
| Total number of observations | 115094 | 7737 |
| Number of reflections | 22172 | 1433 |
| <I/σ(I)> | 11.4 | 2.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 | 5.4 |
| CC(1/2) | 0.997 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.02 M HEPES, 0.15 M NaCl, 5% (w/v) glycerol, 0.001 M TCEP |
