9GO7
Structural characterization of sucrose-binding site in human RNase2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.807, 52.576, 56.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.510 - 1.040 |
| R-factor | 0.1233 |
| Rwork | 0.122 |
| R-free | 0.14110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 1.077 |
| High resolution limit [Å] | 1.038 | 1.040 |
| Number of reflections | 46616 | 1209 |
| <I/σ(I)> | 3.75 | |
| Completeness [%] | 83.6 | 30.6 |
| Redundancy | 4 | 2.9 |
| CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1M Sodium formate, 1M Bis-Tris propane, pH 8.2, 50%w/v Polyethylene glycol 3350 |
