9GO1

C1C2 Channelrhodopsin - SMX Dark structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	61.400, 141.700, 94.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.850 - 2.590
R-factor	0.2171
Rwork	0.211
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.919
Data reduction software	CrystFEL (0.9.1)
Data scaling software	CrystFEL (0.9.1.)
Phasing software	PHENIX (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.850	2.690
High resolution limit [Å]	2.590	2.590
Number of reflections	8838	1288
<I/σ(I)>	10.7	1.05
Completeness [%]	67.7
Redundancy	415.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100 mM sodium citrate (pH 6.0), 30% PEG500DME, 100 mM MgCl2, 100 mM NaCl, and 100 mM (NH4)2SO4.