9GNT
Structural characterization of a glucose-binding site in human RNase2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.900, 53.024, 56.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.710 - 1.310 |
| R-factor | 0.1652 |
| Rwork | 0.163 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.710 | 1.357 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Number of reflections | 25888 | 1296 |
| <I/σ(I)> | 1.71 | |
| Completeness [%] | 90.9 | 46.5 |
| Redundancy | 4.3 | 5.2 |
| CC(1/2) | 0.994 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.05M Citric acid, 0.05M Bis-Tris propane pH 5.0, 16%w/v Polyethylene glycol 3350 |
