9GNJ

Crystal structure of a PP2A B56gamma double phosphorylated BRCA2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	53.300, 108.120, 120.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 2.850
R-factor	0.1984
Rwork	0.197
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	2.952
High resolution limit [Å]	2.850	2.850
Number of reflections	16827	21864
<I/σ(I)>	20.82
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	1.000	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% de PEG3350, 100mM Bis-Tris propane, 200 mM de Sodium Citrate