9GN2

Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Complex with ribose and cytosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	120
Detector technology	PIXEL
Collection date	2024-06-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976269
Spacegroup name	I 21 3
Unit cell lengths	148.981, 148.981, 148.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.820 - 2.410
Rwork	0.190
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.820	2.500
High resolution limit [Å]	2.410	2.410
Rmerge	0.070	1.650
Rpim	0.020	0.370
Number of reflections	21394	2231
<I/σ(I)>	35	3
Completeness [%]	100.0	100
Redundancy	40.5	40.6
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	35% tascimate pH 7.0