9GM2
Human SMUG1 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.6702 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.072, 84.366, 88.259 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 72.045 - 2.100 |
| Rwork | 0.207 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.045 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 56982 | 2865 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.994 | 0.328 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M diammonium hydrogen citrate pH 4.9, 60% w/v PEG 3350 |
