9GLY
Structure of an ancestral laccase from Agaricales fungi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.660, 96.540, 57.058 |
| Unit cell angles | 90.00, 93.38, 90.00 |
Refinement procedure
| Resolution | 48.270 - 1.540 |
| R-factor | 0.1683 |
| Rwork | 0.168 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.151 | 1.759 |
| Rmeas | 0.179 | |
| Number of reflections | 67790 | 3302 |
| <I/σ(I)> | 9.7 | 1.2 |
| Completeness [%] | 96.4 | 95 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.998 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1 M TRIS-HCl at pH 7.0 containing 20% PEG 3000 and 0.2 M calcium acetate |
