9GLT

Crystal Structure of Yeast Ubc13 C87E

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	298
Detector technology	PIXEL
Collection date	2024-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87313
Spacegroup name	P 1 21 1
Unit cell lengths	25.603, 127.663, 42.330
Unit cell angles	90.00, 96.21, 90.00

Refinement procedure

Resolution	42.080 - 1.450
Rwork	0.191
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.758
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.080	1.502
High resolution limit [Å]	1.450	1.450
Rmeas	0.065	0.580
Number of reflections	46789	4758
<I/σ(I)>	11.87	2.6
Completeness [%]	98.1	99.69
Redundancy	2.8	2.9
CC(1/2)	0.996	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M sodium cacodylate, pH 6.5, 25% PEG 4000