9GLQ

Crystal structure of p73 tetramerisation domain in complex with darpins 1800

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	I 2 2 2
Unit cell lengths	62.763, 94.816, 96.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.100
R-factor	0.2043
Rwork	0.202
R-free	0.24782
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.053	0.913
Rmeas	0.060	1.056
Rpim	0.022	0.375
Number of reflections	17152	1653
<I/σ(I)>	15.1	2
Completeness [%]	99.9
Redundancy	7.5	7.7
CC(1/2)	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	30% PEG2000MME, 0.15M potassium bromide