9GLN

Crystal Structure of UFC1 C116E

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2024-01-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	P 41 21 2
Unit cell lengths	46.727, 46.727, 143.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.430 - 1.920
Rwork	0.182
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.538
Data reduction software	autoPROC
Data scaling software	autoPROC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.430	1.989
High resolution limit [Å]	1.920	1.920
Rmeas	0.055	0.217
Number of reflections	12645	1247
<I/σ(I)>	9.8	3.7
Completeness [%]	98.0	100
Redundancy	2	2
CC(1/2)	0.998	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Bis-Tris, pH 5.5, 1.8 M smmonium sulfate.