9GLK

Crystal Structure of UFC1 E149I

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-08-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54187
Spacegroup name	P 41 21 2
Unit cell lengths	47.300, 47.300, 143.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.470 - 2.030
Rwork	0.208
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.571
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.470	2.103
High resolution limit [Å]	2.030	2.030
Rmeas	0.190	0.959
Number of reflections	19946	1471
<I/σ(I)>	10.38	1.75
Completeness [%]	99.5	99.8
Redundancy	3.38	2.18
CC(1/2)	0.973	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M Bis-Tris pH 5.5, 2.0M Ammonium sulfate