9GL1
Crystal Structure of Acetylpolyamine aminohydrolase (ApaH) from Legionella cherrii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.564, 113.564, 90.426 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.200 - 2.400 |
| R-factor | 0.2388 |
| Rwork | 0.237 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS (Jun 30, 2023 BUILT=20230630) |
| Data scaling software | XDS (Jun 30, 2023 BUILT=20230630) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 30383 | 3745 |
| <I/σ(I)> | 12.99 | |
| Completeness [%] | 95.4 | 74 |
| Redundancy | 1.4 | |
| CC(1/2) | 0.998 | 0.041 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1 M (NH4)2SO4 5% (w/v) PEG400 0.1 M Na-MES pH 6.5 |
