9GL0

Crystal Structure of Acetylpolyamine aminohydrolase (ApaH) from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976300
Spacegroup name	P 41 2 2
Unit cell lengths	109.121, 109.121, 400.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.960 - 2.700
R-factor	0.2441
Rwork	0.240
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220820)
Data scaling software	XDS (VERSION Jan 10, 2022 BUILT=20220820)
Phasing software	PHASER (v3.60.0)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	2.890
High resolution limit [Å]	2.700	2.700
Rmeas	0.161
Number of reflections	66768	9462
<I/σ(I)>	12.38	0.59
Completeness [%]	98.0	87.3
Redundancy	24.7
CC(1/2)	0.999	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.1M calcium acetate 0.1M Na-HEPES pH 7.5, 18% (w/v) PEG8000