9GII

Jumonji domain-containing protein 2A with crystallization epitope mutation R913A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 41 3 2
Unit cell lengths	133.870, 133.870, 133.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.375 - 1.700
Rwork	0.198
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.459
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.77))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.375	1.740
High resolution limit [Å]	1.700	1.700
Number of reflections	22799	1650
<I/σ(I)>	22
Completeness [%]	100.0
Redundancy	39.1
CC(1/2)	0.994	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulfate, 0.1M bis-tris pH 6.5