9GHL
Fe(II)-2-oxoglutarate-dependent pseudomonal iron uptake factor C in complex with malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.843, 80.912, 88.701 |
| Unit cell angles | 62.89, 62.90, 60.03 |
Refinement procedure
| Resolution | 59.580 - 2.000 |
| R-factor | 0.1833 |
| Rwork | 0.183 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.593 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.580 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.616 |
| Rmeas | 0.103 | 0.718 |
| Rpim | 0.053 | 0.366 |
| Number of reflections | 108227 | 5273 |
| <I/σ(I)> | 9.1 | 0.8 |
| Completeness [%] | 96.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.995 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 0.2 M Imidazole malate, pH 5.5 33 % v/v PEG 600 |
