9GH3

pleckstrin homology domain interacting protein with crystallization epitope mutations L1408N:R1409E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9282
Spacegroup name	C 1 2 1
Unit cell lengths	83.770, 26.980, 55.830
Unit cell angles	90.00, 100.56, 90.00

Refinement procedure

Resolution	41.210 - 1.190
Rwork	0.179
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.041
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.77))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.210	1.190
High resolution limit [Å]	1.160	1.160
Number of reflections	40440	2175
<I/σ(I)>	10.5
Completeness [%]	94.7
Redundancy	2.7
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3000 0.1M citrate pH 5.5