9GFP
The crystal structure of CsvR from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.613, 77.601, 184.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.320 - 3.030 |
| R-factor | 0.21641 |
| Rwork | 0.212 |
| R-free | 0.29781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.608 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 3.285 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.175 | 2.748 |
| Rmeas | 0.183 | 2.861 |
| Rpim | 0.051 | 0.791 |
| Number of reflections | 8763 | 438 |
| <I/σ(I)> | 10.1 | 1.2 |
| Completeness [%] | 69.8 | 16.6 |
| Redundancy | 12.9 | 13 |
| CC(1/2) | 0.998 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 %w/v PEG3350 0.15 M DL-malic 7 pH 6 %w/v Glycerol |
