9GFL
Crystal structure of ASO binding Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999859 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.171, 59.615, 62.692 |
| Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
| Resolution | 16.900 - 1.680 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.380 | 1.780 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.055 | 0.830 |
| Number of reflections | 53352 | 8399 |
| <I/σ(I)> | 7.32 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.43 | |
| CC(1/2) | 0.999 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.0, 20% PEG8000 |
