9GFH
BCR Fab from the subset 1 chronic lymphocytic leukaemia case P1173
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 125.416, 128.509, 134.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.770 - 2.720 |
| R-factor | 0.2151 |
| Rwork | 0.214 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.645 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.770 | 2.020 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rpim | 0.034 | 0.399 |
| Number of reflections | 123151 | 6158 |
| <I/σ(I)> | 12.4 | 1.7 |
| Completeness [%] | 96.2 | 81.5 |
| Redundancy | 13.4 | |
| CC(1/2) | 0.998 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M citric acid pH 5 20% PEG 6,000 |
