9GFG
BCR Fab from the subset 1 chronic lymphocytic leukaemia case P3129
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.696, 87.891, 115.528 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 80.600 - 2.300 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.634 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.200 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rpim | 0.032 | 0.830 |
| Number of reflections | 120564 | 8714 |
| <I/σ(I)> | 10 | 0.6 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.999 | 0.238 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M sodium potassium tartrate pH 7, 0.1 M magnesium chloride, 20% PEG 3,350 |
