9GFD
Crystal structure of ASO binding Fab fragment with ASO139
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.885603 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.824, 58.044, 62.472 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 42.620 - 1.430 |
| R-factor | 0.1837 |
| Rwork | 0.183 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.290 | 1.530 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.058 | 0.810 |
| Number of reflections | 82939 | 15174 |
| <I/σ(I)> | 9.72 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.14 | |
| CC(1/2) | 0.997 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.0, 20% PEG8000 |
