9GFC
HDM2 complexed with stapled peptide-like ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-27 |
| Detector | DECTRIS EIGER2 R 1M |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.628, 40.736, 78.177 |
| Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
| Resolution | 28.130 - 2.501 |
| R-factor | 0.2659 |
| Rwork | 0.263 |
| R-free | 0.31750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.890 |
| Data reduction software | CrysalisPro (v171.42) |
| Data scaling software | CrysalisPro (v171.42) |
| Phasing software | PHASER (v2.8.2) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.130 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.113 | 0.367 |
| Number of reflections | 13311 | 1375 |
| <I/σ(I)> | 5.69 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | Ammonium Sulfate 2.5M + Tris 0.1M pH8.5 |
