9GF5

CRYSTAL STRUCTURE OF COMPLEX OF ASO BINDING FAB FRAGMENT IN COMPLEX WITH ASO980

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999991
Spacegroup name	C 1 2 1
Unit cell lengths	126.615, 59.604, 62.508
Unit cell angles	90.00, 94.34, 90.00

Refinement procedure

Resolution	53.900 - 1.570
R-factor	0.1901
Rwork	0.189
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.900	1.660
High resolution limit [Å]	1.570	1.570
Rmerge	0.038	0.630
Number of reflections	63077	8352
<I/σ(I)>	10.44
Completeness [%]	97.2
Redundancy	3.19
CC(1/2)	0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.0, 20% PEG8000