9GEA
Structure of UP1 S4DS6D phosphomimetic mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-07-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.193, 43.709, 55.808 |
Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
Resolution | 55.690 - 1.890 |
R-factor | 0.1826 |
Rwork | 0.181 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.964 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.690 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 14914 | 973 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 100.0 | |
Redundancy | 1.96 | |
CC(1/2) | 0.990 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M Tris, 25 % PEG-4000, 20 % 2-Methyl-2,4-pentanediol |