9GEA

Structure of UP1 S4DS6D phosphomimetic mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	38.193, 43.709, 55.808
Unit cell angles	90.00, 93.69, 90.00

Refinement procedure

Resolution	55.690 - 1.890
R-factor	0.1826
Rwork	0.181
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.690	1.930
High resolution limit [Å]	1.890	1.890
Number of reflections	14914	973
<I/σ(I)>	6.7
Completeness [%]	100.0
Redundancy	1.96
CC(1/2)	0.990	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1 M Tris, 25 % PEG-4000, 20 % 2-Methyl-2,4-pentanediol