9GDJ

C-Methyltransferase SgMT from Streptomyces griseoviridis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-30
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.8856
Spacegroup name	P 41 21 2
Unit cell lengths	123.920, 123.920, 126.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.430 - 1.470
R-factor	0.1477
Rwork	0.147
R-free	0.17490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.430	1.510
High resolution limit [Å]	1.470	1.470
Rmeas	0.113	3.287
Number of reflections	166186	12132
<I/σ(I)>	11.65	0.78
Completeness [%]	100.0	100
Redundancy	13.2	13.17
CC(1/2)	0.998	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	sodium citrate, PEG 4000, 2-propanol, glycerol, hexammine cobalt(III) chloride, S-adenosyl homocysteine, cyclo(Trp-Trp)