9GD5
Crystal structure of apo TRIM24 PHD-BRD in C121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.900, 36.907, 129.363 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 44.450 - 1.681 |
| Rwork | 0.210 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.602 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.77)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmeas | 0.144 | |
| Number of reflections | 43525 | 1485 |
| <I/σ(I)> | 1.97 | |
| Completeness [%] | 95.7 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M ammonium sulfate, 0.1M HEPES (pH 7.9), 2% PEG400, 8% glycerol |
