9GD4

Crystal structure of septin complex Shs1-Cdc12-Cdc3-Cdc10 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873129
Spacegroup name	P 1 21 1
Unit cell lengths	98.205, 47.845, 148.885
Unit cell angles	90.00, 103.29, 90.00

Refinement procedure

Resolution	23.490 - 2.039
R-factor	0.218
Rwork	0.216
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	XDS (Jun 30, 2023 (BUILT 20230630))
Data scaling software	STARANISO (2.3.94 (20230525))
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.900	2.355
High resolution limit [Å]	2.039	2.039
Rmerge	0.304	1.567
Rmeas	0.338	1.753
Rpim	0.145	0.774
Number of reflections	41902	2095
<I/σ(I)>	5.2	1.9
Completeness [%]	89.3	61.7
Redundancy	5.4	5
CC(1/2)	0.973	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	17.5 % PEG 5000, 0.2 M ammonium sulphate, 0.1 M BIS-TRIS pH 6.5